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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.1: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Adjustment of pulsed laser radiation for stroboscopic experiments — •Benedikt Kamp¹, Tobias Löffler¹, Julia Kristin², and Mathias Getzlaff¹ — ¹Institute of Applied Physics, Heinrich-Heine-Universität Düsseldorf — ²Hals-Nasen-Ohrenklinik, Universitätsklinikum Düsseldorf

This contribution aims to adjust the pulsed laser radiation of a fluorescence microscope depending on an electrical voltage. Through this a stroboscopic effect can be achieved and the periodic movement of an observed probe can be researched in even more detail. The described microscope already has an integrated array of shutters, which are operated by TTL-signals. First the incoming alternating voltage is regulated in amplitude and filtered. The resulting sinewave is converted into a rectangular voltage of 5V by a comparator, so that it can be used as TTL-signal. This signal runs through monostable multivibrators, in which the time ratio of the logic "high" and "low" can be changed with external potentiometers without changing the frequency. It results in a device that can change the time length and phase of the "high", in order to be able to control the shutters accordingly. Furthermore the device possesses overvoltage protection, so that the microscope cannot suffer damage. In future, for even better usage of the stroboscopic effect, a counter can be implemented for limiting the frequency the shutters have to work at.