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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.5: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Photothermal detection of single gold nanoparticles in living fibroblasts — •Alice Abend, Romy Schachoff, and Frank Cichos — Universität Leipzig

Live cell bioimaging allows for the observation of cellular processes and their dynamics and provides insight into functions of cells such as metabolism, replication and movement. Modern nanotechnology enables manufacturing of nanometer sized objects with tailored optical properties and specific functionalization which turns them into ideal optical probes for several imaging techniques. We deliver gold nanoparticles (AuNPs) to the cells (NIH/3T3) as they are photostable and allow for long term imaging and seem to be less toxic to living organisms in comparison to semiconductor quantum dots. Our imaging method, photothermal optical microscopy, provides sensitive detection of AuNPs and is therefore suitable to prove the presence of AuNPs in the fibroblasts' interior. As photothermal microscopy is based on heating of the contrast agent, AuNPs can double as heat sources for inducing local intracellular temperature fields which could be useful to manipulate cellular functions such as protein synthesis, metabolism processes and cell motility.