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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.6: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Quantitative Phase Microscopy with a Single Shot Measurement Technique — •Tobias Neckernuss¹, Jonas Pfeil¹, Christoph Koch², and Othmar Marti¹ — ¹Institute of Experimental Physics, Ulm University — ²Department of Physics, Humboldt University of Berlin

In cellular biophysics, there are numerous interesting questions concerning the height of cells under different conditions. However, using classical microscopy techniques recovering the height of an object is related to determine the phase change of the light and is a tough task. Most of the common phase retrieval methods require a stack of images and hence are limited to nonmoving cells since it takes several seconds to capture all images. A second possibility is interferometry where the most critical point is the mechanical stability of the setup so that it is often not applicable in normal laboratories. We introduce an inline holography setup where we use the transport of intensity equation (TIE) to recover the optical path length of the sample from two differently focused pictures. The pictures are taken simultaneously which means that camera speed is the only limitation and therefore changes up to 100Hz can be resolved. Several refinements had to be applied to the reconstruction algorithm in order to get it working since it was originally designed for electron microscopy using more than two pictures. We use a partially coherent LED lamp to avoid common artifacts emerging from laser illumination. Data of polystyrene beads on surfaces as well as of adherent cells in medium is shown. We will compare the reconstructed height with the real ones measured by AFM.