Dresden 2017 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 8: Posters - Bioimaging and Spectroscopy

BP 8.7: Poster

Montag, 20. März 2017, 17:30–19:30, P3

Fluorescent Gold Nanoparticles on/in Cells Visualized by Fluorescence-Lifetime Imaging Microscopy — •Marina Mutas, Tim Hadler, Christian Strelow, Tobias Kipp, and Alf Mews — Institut für Physikalische Chemie, Universität Hamburg

Fluorescence-Lifetime Imaging Microscopy (FLIM) is a powerful method to discriminate emitters with different fluorescence lifetimes. Gold nanoclusters with mercaptoundecanoic acid as stabilizing ligand (MUA-AuNCs) show a fluorescence emission that peaks at a wavelength around 525 nm with decay times longer than 100 ns. The autofluorescence of biological cells is in the same wavelength region but the fluorescence decay time, which is about 3 ns, is much shorter. We are able to specifically biofunctionalize these MUA-AuNCs with an aptamer which binds to a receptor expressed on the cells' membrane. To get an image of the whole cell we use cross-sectional FLIM scans in axial direction at different heights through the cell. With this technique we are able to visualize specifically bound aptamer-MUA-AuNCs on the cells' membrane using three FLIM methods and reflection images.