Dresden 2017 – scientific programme
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BP: Fachverband Biologische Physik
BP 9: Posters - Cell Mechanics
BP 9.14: Poster
Monday, March 20, 2017, 17:30–19:30, P3
Dynamics of single human cardiomyocytes tracked by endogenous labeling of z-lines using CRISPR/Cas9 — •Daniel Härtter1, Til Driehorst1,2, Malte Tiburcy2, Kengo Nishi1, Wolfram-H. Zimmermann2, and Christoph F. Schmidt1 — 1Georg-August Universität Göttingen, Germany — 2University Medical Center Göttingen, Germany
The dynamics of single cardiomyocytes (CM) may provide insight into cardiac function and dysfunction. The CMs' regulated and coordinated sarcomeric contractility is, however, not fully understood.
We here present a new method using endogenous labeling of the z-lines in living human stem cell-derived CMs using CRISPR/Cas9. We applied methods of micro-contact printing to shape the cells to physiological aspect ratios. Using high-speed confocal microscopy, we imaged the contractile dynamics of individual sarcomeres with high spatial and temporal resolution. We developed a method to track z-lines over time and to analyze their dynamics. We utilize approaches from nonlinear dynamic systems theory to study phase-coherence and synchronization. We could show that the phase coherence of sarcomeres strongly depends on the elastic properties of the underlying substrate. We further investigated the effects of drug interference on the sarcomeric level.