Dresden 2017 – scientific programme

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BP: Fachverband Biologische Physik

BP 9: Posters - Cell Mechanics

BP 9.2: Poster

Monday, March 20, 2017, 17:30–19:30, P3

Development of a mechanically stable cell stretcher for measuring the influence of external strain on cell mechanics with the AFM — •Fabian Port, Patrick Paul, and Kay-E. Gottschalk — Institute of Experimental Physics, Ulm University

In the last few decades the correlation between cell mechanics and different physiological or pathophysiological conditions, like stem cell differentiation [1] or cancer [2], has been a growing aspect of biophysical research. To understand the underlying mechano-chemical feedback cycles, it is important to understand the mechanical properties of cells under varying conditions. Such conditions could be the effect of strain on cells, which is particularly important for a variety of cell types like endothelial cells in the lung, in arteries or in the bladder. The impact of such conditions on the cell mechanics is not yet well understood on the cellular and subcellular level. For a detailed analysis of the cells response to stretch, we present here a self-developed cell stretching device, which is compatible with correlative AFM and FLIM Measurements.

[1] Suresh, S., Spatz, J., Mills, J. P., Micoulet, A., Dao, M., Lim, C.T.,and Seufferlein, T. (2005). Connections between single-cell biomechanics and human disease states: gastrointestinal cancer and malaria. Acta Biomaterialia, 1(1), 15-30.

[2] Sokolov, I. (2007). Atomic force microscopy in cancer cell research. Cancer Nanotechnology, 1-17.