Dresden 2017 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 56: Hydrogels and Microgels I

CPP 56.3: Talk

Thursday, March 23, 2017, 11:00–11:15, ZEU 260

Cell-free protein synthesis and enzymatic cascades in polymer microgels — •Julian Thiele — Leibniz-Institut für Polymerforschung Dresden e.V., Leibniz Research Cluster, Germany

Microfluidic flow cells provide excellent control over the formation of microemulsions, which are widely applied as templates for the fabrication of hydrogel microparticles with defined physicochemical properties and mechanics. The Leibniz Research Cluster group at IPF Dresden utilizes this knowledge to develop biocompatible hydrogel particles as cell-like experimental platform for cell-free biotechnology. Here, spatial organization and confinement of reagents can be easily varied, which directly influence diffusion rates and binding constants, as shown in microgel-based cell-free protein synthesis (Hansen et al. ChemBioChem 2016, 17, 228). Currently, we are extending the application of microfluidically prepared microgels as host for cell-free biosyntheses towards the design of polyketide building blocks in enzymatic cascades.