Dresden 2017 – scientific programme

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CPP: Fachverband Chemische Physik und Polymerphysik

CPP 56: Hydrogels and Microgels I

CPP 56.5: Talk

Thursday, March 23, 2017, 11:45–12:00, ZEU 260

Superresolved fluorescence microscopy to gain structural insights into microgels and other soft matter systems — •Dominik Wöll, Alex Oppermann, Arjan Gelissen, Oleksii Nevskyi, and Walter Richtering — Institute of Physical Chemistry, RWTH Aachen University, Germany

The elucidation of the structure of compartimentalized microgels and microgel structures is challenging since they do not possess strong contrast for electron microscopy, and since classical fluorescence microscopy fails due to the fact that their structures are significantly smaller than the diffraction limit of optical light. However, modern superresolved fluorescence microscopy methods such as dSTORM (direct stochastic optical reconstruction microscopy) reach resolutions down to 10-30 nm and, therefore, are highly suited to fill this gap of structural in situ imaging. In this contribution, the success of these methods to visualize microgels and other soft matter systems and their application in determining the spatial distribution of implemented functionalities will be demonstrated.