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DS: Fachverband Dünne Schichten

DS 44: Postersession II

DS 44.44: Poster

Donnerstag, 23. März 2017, 17:00–19:00, P1C

X-Ray Reflectivity Investigation of Structure and Kinetics of Photoswitchable Lipid Monolayers — •Malte Jacobsen¹, Kuntal Chatterjee¹, Björn Haushahn¹, Chen Shen^1,2, Sven Festersen¹, Jonas Warias¹, Benjamin Runge¹, Franziska Reise³, Thisbe Lindhorst³, Beate Maria Klösgen², Olaf Magnussen^1,4, and Bridget Murphy^1,4 — ¹Institute for Experimental and Applied Physics, CAU Kiel, Germany — ²SDU Odense, Denmark — ³Institute of Organic Chemistry, CAU Kiel, Germany — ⁴Ruprecht Haensel Laboratory, CAU Kiel, Germany

The mechanical and dynamic properties of phospholipid membranes are important for biological functions such as switching of embedded proteins. To investigate these biologically relevant properties we study model systems in which amphiphilic photoswitchable glycoconjugates have been integrated into biomimetic phospholipid membranes. The structure and kinetics of the switching process in a lipid Langmuir film are measured using Langmuir isotherms and in situ X-ray reflectivity at LISA diffractometer P08, PETRA III. We have modified glycolipids to contain an azobenzene photoswitch between the chain and head group and successfully embedded them in a monolayer of DPPC. We can reversibly change the azobenzene-glycolipid orientation between trans- and cis-conformation by illumination with UV and blue light. Strong changes in membrane conformation upon switching have been observed, and a critical point has been discovered above which the membrane expands rather than compresses upon switching. This work is funded by SFB 677 and BMBF 05K13FK2.