Dresden 2017 – scientific programme

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MA: Fachverband Magnetismus

MA 64: Poster 1

MA 64.68: Poster

Friday, March 24, 2017, 09:30–13:00, P2-EG

Extraction of Magnetic Nanoparticles from Magnetotactic Bacteria — •Lea Schwengels, Nadja Juric, Maryam Youhannayee, and Mathias Getzlaff — Institute of Applied Physics, Heinrich-Heine-Universität, Düsseldorf, Germany

Nanoparticles attract fascinating attention because of their capability in using for biological and medical application and research. Therefore, it seems to be promising to apply magnetic nanoparticles for different cancer treatments like hypothermia. Our research deals with the extraction and separation of chains of magnetite nanoparticles from magnetotactic bacteria (MTB) called Magnetospirillum magnetotacticum (MS-1) and subsequently the extraction of single magnetic nanoparticles from these obtained magnetosomes. Cultured Magnetospirillum magnetotacticum were purchased from DSMZ as an actively growing culture in specific liquid medium. In our research we use two different techniques to separate the nanoparticles from bacteria, ultrasonic and applying a combination of lysozyme and EDTA (Ethylenediaminetetraacetic). The combination of using lysozyme and EDTA represents a useful method to extract the magnetosome chains from our enriched bacteria without affecting the magnetite nanoparticle and its membrane. Using ultrasonic cause the lysis of bacteria and separation of magnetosome chains who are still surrounded by cell residues. Transmission electron microscopy has been applied in order to characterize the morphological structure of magnetite nanoparticles like shape and size.