Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 1: Protein Structure and Dynamics
BP 1.3: Vortrag
Montag, 12. März 2018, 10:15–10:30, H 1028
Dramatic influence of anisotropic interaction and shape on short-time protein diffusion — Jin Suk Myung1, •Felix Roosen-Runge1, Roland G. Winkler2, Gerhard Gompper2, Peter Schurtenberger1, and Anna Stradner1 — 1Division of Physical Chemistry, Lund University, Sweden — 2Theoretical Soft Matter and Biophysics, Forschungszentrum Jülich, Germany
Diffusion of proteins in cells is an essential process, strongly influencing the cellular machinery through numerous processes such as signal transmission or reactions between proteins. We present a combined experimental and computational study on effects of anisotropic interactions and shape on the initial step of structural relaxation on nearest-neighbor distance, i.e. short-time cage diffusion. Using neutron spin echo spectroscopy in crowded solutions of crystallin proteins, cage diffusion for α crystallin follows predictions for hard spheres, while the cage diffusion of the weakly attractive γ crystallin shows a dramatical slowing down at comparably low volume fractions [1]. In mesoscale hydrodynamic simulations employing multiparticle collision dynamics (MPC), we observe a significant dynamical slowing down due to attractions, which is strongly enhanced due to anisotropy in protein interactions [1] and shape [2]. These results particularly demonstrate that simplistic spherical models for globular proteins can be severely misleading when studying effects of crowding on structural relaxation and diffusion.
[1] S Bucciarelli, JS Myung et al. Sci. Adv. (2016) 2:e1601432
[2] JS Myung, F Roosen-Runge et al. in preparation