Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.15: Poster
Montag, 12. März 2018, 17:30–19:30, Poster C
Cellular Forces under Altered Gravity Conditions — •Julia Eckert1, 2, 3, Stefano Coppola2, Thomas Schmidt2, Lukas M. Eng1, Robert Lindner3, and Jack J.W.A. van Loon3, 4 — 1School of Science, Department of Physics, Technische Universität Dresden, Dresden, Germany — 2Physics of Life Processes, Leiden Institute of Physics, Leiden University, Leiden, The Netherlands — 3Life & Physical Science, Instrumentation and Life Support Laboratory (TEC-MMG), ESA/ESTEC, Noordwijk, The Netherlands — 4VU Medical Center/ACTA, Amsterdam, The Netherlands
During future long-term space missions, the human organism will be exposed to microgravity and other gravitational levels for extended periods of time. Hence, it is of vital importance to develop a basic understanding of mechanobiology and mechanosensing under such conditions.
Here, we introduce a measuring approach based on a micropillar array technology. Different cell types, like bone and fibroblast cells, were placed on polydimethylsiloxane pillars with controlled stiffness allowing to examine cell traction forces. Further, they were exposed to altered gravity in the Large Diameter Centrifuge and in the Random Positioning Machine of the European Space Agency in The Netherlands. We found that cells respond to the new gravitational environment and their behavior depend on g-force into or away from the pillar array, substrate stiffness and their cell type. Our approach is suitable to study biological response to changing gravity conditions at the cellular level.