Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.2: Poster
Montag, 12. März 2018, 17:30–19:30, Poster C
Comparison of the cytoskeleton of squamous cells using fluorescence microscopy — •Mona Plettenberg1, Maja Strugacevac1, Constanze Wiek2, Julia Kristin2, Marcel Glaas2, Jörg Schipper2, and Mathias Getzlaff1 — 1Institute of Applied Physics, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany — 2Düsseldorf University Hospital, Department of Othorinolaringology, Moorenstrasse 5, 40225 Düsseldorf, Germany
Our group's aim is to investigate the mechanical properties of benign and malign squamous cells. The fluorescence microscopy is one of the group's essential experimental techniques to gain new knowledge.
The cell's mechanical properties are mainly determined by the cytoskeleton. Especially the microtubule and actin filaments are key features for the cell's elasticity. In cancerous squamous cells, the cytoskeleton structures are changing. To investigate the differences, we compared the cytoskeleton of four cell lines extracted from tumors of different states in the head-neck area. By staining the cytoskeleton filaments with SiR-Actin and SiR-Tubulin, they could be observed under the fluorescence microscope. For this purpose, we optimized the staining process for our used cell lines.
The higher the tumor state is, the more the cytoskeleton is unorganized and the cells show more motility characteristics. We also could observe a decline of the actin-skeleton as well as of the microtubules. These facts lead us to the assumption that our investigated cells are more elastic in a higher tumor state.