Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Postersession II

BP 10.23: Poster

Montag, 12. März 2018, 17:30–19:30, Poster C

Near Real Time Analysis of Stress Fiber Formation in Stem Cells — •Lara Hauke, Carina Wollnik, and Florian Rehfeldt — University of Göttingen, Third Institute of Physics - Biophysics

Human mesenchymal stem cells (hMSC) can be directed to differentiate into various lineages by different matrix elasticities. While changes in lineage specific protein expression occur over a period of days to weeks, significantly different structures of stress fibers are observable within the first 24 hours of plating [1] quantified by an order parameter S. With our massively parallel live-cell imaging set-up we record cells under physiological conditions (37 ^∘C, 5 %CO2) over a period of 24-48 hours to obtain a statistically sufficiently large data set. We aim for a full representation of filament processes over time and space allowing for statistical analysis. This unbiased classification will be represented by persistence in space and time and potential cross-talk with other cytoskeletal components. For this we developed the *FilamentSensor* [2,3] a freely available tool for near real-time image analysis of stress fibers. We present experimental data where we can distinguish the development of hMSCs on 1 kPa, 10 kPa and 30 kPa elastic substrates with 99 % confidence and are working on single filament tracking and better analysis of orientation fields. References: [1]A. Zemel, et al., Nat. Phys., 2010. [2]www.filament-sensor.de [3]B. Eltzner, et al., PLoS One, 2015.