Berlin 2018 – scientific programme
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.24: Poster
Monday, March 12, 2018, 17:30–19:30, Poster C
Elastic Response of Epithelial Model Tissues in Deformation Experiments — •Simone Gehrer1, Sara Kaliman1, Damir Vurnek1, Maryam Aliee1, Shuqing Chen2, Andreas Maier2, Diana Dudziak3, Rudolf Merkel4, and Ana-Sunčana Smith1,5 — 1PULS Group, Institute for Theoretical Physics I, FAU Erlangen — 2Pattern Recognition Lab, Department of Computer Science 5, FAU Erlangen — 3Dermatology, Universitätsklinik Erlangen — 4ICS-7: Biomechanics, Forschungszentrum Jülich — 5Division of Physical Chemistry, IRB Zagreb
Epithelial tissues act as barriers between different tissue types and form boundaries of the majority of organs. They are often exposed to mechanical stress to which they quickly respond by changing shape and internal organization on short time scales. On longer time scales, stress can be released by proliferation and growth.
To investigate the response of MDCK II tissues grown on PDMS substrates, we expose the cell-colonies to an uniaxial stress (10 – 30%) using a stretching device. The resulting changes are monitored for minutes to days by phase-contrast and confocal microscopy. The reversible changes in the overall shape of the cell confirm that on short time scales the tissue responds as an elastic material. This is furthermore confirmed by analysing the morphology and connectivity of individual cells before and after stress. On longer time scales, we find that the growth rate of the colony is affected by the continuous deformation, while after 24 – 48h the tissue adapts and resumes the morphological and topological characteristics as the control.