Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Postersession II

BP 10.36: Poster

Montag, 12. März 2018, 17:30–19:30, Poster C

3D collective migration in cancer spheroids and during invasion — •Swetha Raghuraman and Timo Betz — Institute of Cell Biology, ZMBE, University of Münster, Von-Esmarch-Straße 56,48149 Münster

Cancer cells move collectively in order to migrate and overcome space constrictions and geometric obstacles. This movement is characterized by cell-cell adhesion differences, the extracellular matrix or cell-substrate interactions, as well as intercellular cross-talks during invasion, morphogenesis and wound healing. The mechanisms and the physics behind coordinated behavior of cells have been studied recently at the 2D level, and in-vivo tissue homeostasis. However, due to the challenges involved in reproducing and imaging tumor spheroids, collective motion of 3D cancer invasion remains a complex task. With the use of Light Sheet Microscopy, we have been able to record cancer spheroids with fluorescently marked nuclei over several days at sub-cellular resolution. 3D particle tracking of several thousand cells allows well defined velocity correlations and density fluctuation measurements within the spheroid, and at the cell invading a collagen matrix. By combining these parameters we will test the hypothesis whether cancer cell migration in 3D is physically similar to active jamming, as recently suggested in 2D situations.