Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Postersession II

BP 10.3: Poster

Montag, 12. März 2018, 17:30–19:30, Poster C

FRAP traces of immobile self-assembled complexes from Monte-Carlo simulations — •Justin Grewe^{1, 2} and Ulrich S. Schwarz^{1, 2} — ¹Institute for Theoretical Physics, Heidelberg — ²Bioquant, Heidelberg

Non-muscle myosin II plays an important role in many essential cellular processes, including adhesion, migration and cytokinesis. Because myosin II is a non-processive motor, it cannot generate appreciable levels of force by itself, but needs to work in larger ensembles. In non-muscle cells, it assembles into myosin II minifilaments, which are approximately 300 nm large and contain around 30 myosin II molecules. As shown by experimental FRAP studies, this supramolecular complex is very dynamic, exchanging myosin monomers with a typical half time of 70 seconds. Using Monte-Carlo methods, we study the interplay between assembly and force generation in a spatial model for minifilaments. Our simulated FRAP-traces show the signature of different time scales, in contrast to the standard analysis of experimental FRAP-traces, which uses only one time scale.