Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Postersession II

BP 10.51: Poster

Montag, 12. März 2018, 17:30–19:30, Poster C

Single cell migration and transitions to different substrates on micro patterns — •Christoph Schreiber, Felix J. Segerer, and Joachim O. Rädler — Faculty of Physics and Center for NanoScience, LMU München

When cells migrate in the body, for example during cancer metastasis, cells are facing different extra cellular matrix (ECM) proteins that can influence the cell migration behavior. To study the effects of different ECM proteins, standardized experimental conditions and suitable metrics to characterize cell motility are needed. We use micro-contact printed stripes or rings to get race tracks for cells with defined protein coatings. The tracks constrict cells to move only in 1D, which simplifies the analysis of the movement.

We find bimodal migration behavior with states of directional migration (run states) and reorientation (rest states).[1] We extract characteristic persistence times, which, in combination with the velocity of cells in the run state, provide a set of parameters quantifying cell motion. To be able to study transitions of single cells to different ECM proteins like fibronectin and collagen IV we developed a new patterning technique based on two stamping processes. Thus, transition rates and velocities on different protein coatings can be analyzed. Together this results in a fingerprint-like set of parameters characterizing cell migration that can be used to distinguish cell lines as well as to quantify the effects of motility affecting drugs.

[1] Schreiber et al. Sci. Rep. 6, 26858 (2016)