Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.52: Poster
Montag, 12. März 2018, 17:30–19:30, Poster C
Scanning Ion Conductance Microscopy on osteoblasts with regard to their adhesion on surfaces — •Christian Völkner1, Regina Lange1, MohammadReza Bahrami1, Martina Grüning2, Henrike Rebl2, Ingo Barke1, Barbara Nebe2, and Sylvia Speller1 — 1University of Rostock, Institute of Physics, 18059 Rostock, Germany — 2University Medical Center Rostock, Dept. of Cell Biology, 18057 Rostock, Germany
Our aim is to elucidate mechanisms of initial cell adhesion and migration of osteoblasts (MG63) on material surfaces. To this end we prepare substrates with different properties such as surface charges, polarisability, electric potential and electromagnetic field landscapes. Furthermore we implement an electro-stimulation chamber to apply fields in the quasistatic regime [1]. Our main approach is Scanning Ion Conductance Microscope (SICM), in which a nanopipette is used as a probe and the ion current serves as localized interaction signal. In contrast to other scanning probe methods like Atomic Force Microscopy it allows one to obtain the nanomorphology of the surface of living cells reducing forces between cell and nanoprobe and respective cell responses [2]. Especially, substructures of the migration fronts of adhering osteoblasts are being addressed.
[1] N. W. S. Kam, E. Jan, N. A. Kotov, Nano Lett. 9, 273 (2009)
[2] Y. E. Korchev, C. L. Bashford, M. Milovanovic, I. Vodyanoy, M. J. Lab, Biophys. J. 73, 653 (1997)