Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 10: Postersession II

BP 10.6: Poster

Montag, 12. März 2018, 17:30–19:30, Poster C

Dynamic Actin Structures in Frog Egg Extract — •Jianguo Zhao and Christoph F. Schmidt — Drittes Physikalisches Institut - Biophysik, Fakultät für Physik, Georg-August-Universität Göttingen

The actin cytoskeleton in eukaryotic cells undergoes continuous turnover, which is of crucial importance for various functions, e.g. cell motility and division. While components are constantly exchanged, the concentrations and distributions of components, including monomeric and polymeric actin often maintain a steady-state. On the other hand, cytoskeletal structures are also quite adjustable when needed, and different steady states can be accessed in response to regulatory signals. Given the complexity of cells, it is desirable to be able to study such dynamic rearrangements in reconstituted model systems. We employed water-in-oil emulsion droplets composed of Xenopus laevis egg cytoplasmic extract as a model system. In preliminary experiments, we observed local actin network clusters in the presence of Arp2/3 complex. The size of aggregated clusters was dependent on droplet dimensions, concentration of extract, nucleation protein and MgCl2. Small clusters were quite active and transported stress over a relatively long-distance inside the droplet.