Berlin 2018 – scientific programme
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BP: Fachverband Biologische Physik
BP 10: Postersession II
BP 10.9: Poster
Monday, March 12, 2018, 17:30–19:30, Poster C
Is the size of a cell nucleus an indicator for cancer? — •Lisa Rohde1, Maja Strugacevac1, Nina Bartels1, Constanze Wiek2, Julia Kristin2, Marcel Glaas2, Jörg Schipper2, and Mathias Getzlaff1 — 1Heinrich-Heine-Universität Düsseldorf, Institute of Applied Physics, Düsseldorf, Germany — 2Düsseldorf University Hospital, Department of Otorhinolaryngology
Identifying characteristics of cancer cells is still one of the main topics of recent research. Due to that our group is investigating properties of head and neck squamous cell carcinoma cells and dysplastic oral keratinocytes by fluorescence microscopy. The live cell imaging gives detailed information about single organelles, which is necessary for comparing the cell differences.
The nucleus is involved in the mitosis, contains the DNA and is therefore responsible for important physiological processes. As the mitosis rate is increased cancer cell nuclei hold much more chromatin than healthy cells do. This potential indicator for cancer motivates the comparison of the size of the different cell nuclei.
The cell nucleus is marked with Hoechst 33342, a blue fluorescent staining kit. The used laser scanning fluorescence microscope allows us three-dimensional cell nuclei observation. Different cancer cell lines and oral keratinocytes were compared to investigate whether the size of cell nuclei is an indicator for cancer.