Berlin 2018 – scientific programme

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BP: Fachverband Biologische Physik

BP 11: Bioimaging and Biopspectroscopy I

BP 11.4: Talk

Tuesday, March 13, 2018, 10:30–10:45, H 1028

Observing the Plasmonic Photothermal Effect on Individual BaF3-Cells using Targeted Gold Nanostructures — •Phillip Witthöft, Lisa Prisner, and Alf Mews — Universität Hamburg, Institut für Physikalische Chemie, Hamburg, Deutschland

The exploitation of the plasmonic photothermal effect of gold structures such as gold nanorods for photothermal therapy in cancerous tissue is of great interest for the scientific community. While a wide array of specific and non-specific applications has been developed in the past, further understanding of this process and the parameters involved at the cellular level is of utmost importance for the development of these systems. In our project, we investigate the photothermal effect of gold nanorods in detail on the single cell level. We have developed a method to irradiate only an individual cell with the desired wavelength to induce a plasmonic photothermal effect and successfully observe the reaction of one individual cell to the temperature rise in real time by plotting the color saturation of the cell over time in the presence of trypan blue. We use a targeted delivery system facilitated by the interaction of the Interleukin-6 receptor with the Interleukin-6 specific aptamer AIR-3A and compare the efficiency for plasmonic photothermal therapy of these targeted nanostructures to the efficiency of non-targeted PEG-coated nanostructures. We were able to observe that cells incubated with targeted nanostructures already show their maximum color saturation after half of the time compared to non-targeted nanostructures. Based on our observations we discuss the mechanism and specificity of the photothermal effect on the single cell level.