Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 11: Bioimaging and Biopspectroscopy I

BP 11.7: Vortrag

Dienstag, 13. März 2018, 11:30–11:45, H 1028

CellMOUSE: A novel high throughput real-time measurement method for suspended cells and particles — •Tobias Neckernuss, Daniel Geiger, Jonas Pfeil, Markus Sporer, Stefan Reich, and Othmar Marti — Institute of Experimental Physics, Ulm University

The optical measurement of cells has proven to be a viable tool in biology and in clinical applications. It is used to distinguish different cell types as well as healthy, mutated and dead cells based on parameters like size, shape and morphology. Common methods, based on video microscopy or light scattering, are either limited in throughput, analysis speed or information content.

We present a new optical measurement device, the so called CellMOUSE, that is able to measure suspended cells and particles in real-time with very high throughput of more than 500 cells per second. Measurement quantities like speed, size, morphology and shape of the cell are obtained immediately after passing the detector. In contrast to other techniques, CellMOUSE measures and evaluates each cell individually and the result is not based on the statistics of an ensemble. This is, for instance, important for cell sorting applications. Furthermore, the measurement does not require buffering so that continuous screening of cell properties over an unlimited time span is possible.