Berlin 2018 – scientific programme

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BP: Fachverband Biologische Physik

BP 15: Postersession III

BP 15.11: Poster

Tuesday, March 13, 2018, 14:00–16:00, Poster B

Radio Frequency Detection of Single Particles in Micorfluidic Circuits — •Marcel Hoeft — Center for Hybrid Nanostructures, Hamburg, Deutschland

I will present a method which operates on a different principle than the more common coulter-counting electric-based detection. It is an impedance-based microfluidic circuit that makes use of radio frequency reflectometry to measure the translocation of single particles through a micropore, filled with electrolyte. The device is a coplanar waveguide which lies perpendicular to the direction of flow. As a comparison measurement the change of the ion current is measured during the translocation of named particles. The structure of the cpw is brought onto a coverslip by optical lithography. An ArF excimer laser is used to drill the pore into the coverslip at the sensing region. A focused ion beam is used to reshape the sensing region to ensure that the pore lies within the confinde electric field. An integrated circuit is used to match the device to the 50 ohm. The biggest advantage of this device compared to the other coulter-counting devices is the sampling rate of up to 1 GHz. With such a rate it would be possible to analyze the dynamic of single translocation events of e.g. molecules.