Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.12: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Floating Lipid Bilayers at the Liquid/Liquid interface — •Ernesto Scoppola1, Ignacio Rodriguez-Loureiro1, Samantha Micciulla1, Lucas Kuhrts1, Richard Campbell2, Oleg Konovalov3, Giovanna Fragneto2, and Emanuel Schneck1 — 1Max Planck Institut fur Kolloid und Grenzfkachenforschung, Potsdam, Germany — 2Institut Laue-Langevin, Grenoble, France — 3ESRF, Grenoble, France
Biological membranes are vital components of all living organisms. They form the boundaries between the various compartments of cells and constitute platforms for essential biochemical processes. Structural insight is often a prerequisite to understand the details of these processes. X-ray and neutron reflectometry enable the structural characterization of model biological membranes and of their interactions with a variety of biomolecules. However, when using these approaches, studies on molecules crossing the membrane or deeply penetrating into the bilayer chain region turned out to be difficult, because membrane mobility often suffered from the presence of the solid surface. Here we immobilize lipid membranes near functionalized liquid/liquid (L/L) interfaces. The latter are intrinsically soft, self-healing, defect-free, and enable the manipulation of the interface from both sides. Lipid bilayers were immobilized via vesicle fusion onto oil/water interfaces functionalized with charged lipids and structurally investigated using specular reflectometry. The interaction between the bilayer and the L/L interface was tuned by variation of the ionic strength, as evidenced by a bilayer displacement relative to the interface.