Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.22: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Soft-landing of folded proteins by ES-IBD for imaging — •Sven Szilagyi1, Hannah Ochner1, Lukas Krumbein1, Joseph Gault2, Albert Konijnenberg3, Esther Martin3,4,5, Justin Benesch2, Frank Sobott3,4,5, Carol Robinson2, Sabine Abb1, Stephan Rauschenbach1,2, and Klaus Kern1,6 — 1Max-Planck-Institut für Festkörperforschung, 70569 Stuttgart — 2Department of Chemistry, University of Oxford — 3Department of Chemistry, University of Antwerp — 4Astbury Centre, University of Leeds — 5School of Molecular and Cellular Biology, University of Leeds — 6École polytechnique fédérale de Lausanne, CH-1015 Lausanne
Native electrospray ionization has been shown to successfully bring proteins and protein complexes in their natively folded state into the gas phase, where further analysis by mass spectrometry and ion mobility spectrometry can be performed [1]. However, these methods are not sufficient for determining structural details at the level of imaging techniques such as TEM, AFM, STM or low energy electron holography (LEEH), which require a very clean sample preparation process. Here, we demonstrate the usage of electrospray ion beam deposition (ES-IBD) as a tool for sample preparation of folded proteins for single molecule microscopy [2]. We present examples of successfully deposited molecules imaged using the above techniques and explore the influence of different substrates and environmental conditions.
[1] Nat. Meth., 5(11), 2008, 927-933. [2] Annu. Rev. Anal. Chem. 2016, 9: 16.1-16.26