Berlin 2018 – scientific programme

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BP: Fachverband Biologische Physik

BP 15: Postersession III

BP 15.27: Poster

Tuesday, March 13, 2018, 14:00–16:00, Poster B

An integrated platform for rapid semi-confocal imaging and spatially resolved fluctuation microscopy — •Adal Sabri, Andreas Veres, and Matthias Weiss — Experimental Physics I, University of Bayreuth

Fluorescence imaging is a key method when studying the secret life of cells. Due to the tradeoff between spatial and temporal resolution, rapid, high-quality data acquisition often comes at the cost of complex and technically challenging methods.

Here, we report on a simple line-illumination and slit-filtering approach for the rapid imaging of large specimen (up to 700μm edge length). The technique is about an order of magnitude faster than standard confocal microscopy approaches while maintaining a spatial resolution close to the diffraction limit.

In addition, swift switching to a second excitation/detection path within the same setup allows for performing two-point cross-correlation fluctuation spectroscopy measurements on sub-micron scales. The setup hence allows one to determine local transport coefficients as well as barriers to diffusion and flows in living cells.

Altogether, the setup provides a combination of rapid imaging and the analysis of dynamic intracellular events on subcellular scales.