Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Postersession III

BP 15.29: Poster

Dienstag, 13. März 2018, 14:00–16:00, Poster B

A comparison of quantitative diffusion measurement techniques in a light sheet microscope — •Philipp Struntz and Matthias Weiss — University of Bayreuth, Experimental Physics I , Universitätsstraße 30, 95447 Bayreuth, Germany

Monitoring the diffusion of particles and macromolecules with high spatiotemporal resolution yields important clues about the secret life of biological specimen. Heterogeneous biological samples may feature spatially varying diffusion characteristics while demanding gentle imaging approaches with low phototoxicity to maintain the specimen's viability. Combining single plane illumination microscopy (SPIM) and fluorescence correlation spectroscopy (FCS) provides a versatile and gentle measurement technique for spatially parallelized diffusion measurements, even in fragile developmental model systems like Caenorhabditis elegans [1]. In order to compare SPIM-FCS to complementary techniques like Single Particle Tracking (SPT) and Differential Dynamic Microscopy (DDM), we have used in-vitro systems of fluorescently labeled particles in aequous solutions. While SPIM-FCS and SPT basically report on a particle's mean square displacement, DDM is a light scattering technique based on the analysis of power spectra of difference images. All three techniques were implemented on the same custom-made SPIM setup. As a result, we observed that each technique has certain strengths and weaknesses regarding sample properties and setup characteristics.

[1] P. Struntz & M. Weiss, J. Phys. D 49, 044002 (2016).