Berlin 2018 – scientific programme
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.33: Poster
Tuesday, March 13, 2018, 14:00–16:00, Poster B
High-speed imaging of rotational diffusion of a gold nanorod on a supported lipid bilayer — •Mahdi Mazaheri1 and Vahid Sandoghdar1,2 — 1Max Planck Institute for the Science of Light, Erlangen, Germany. — 2Department of Physics, Friedrich Alexander University (FAU) Erlangen-Nürnberg, Erlangen, Germany.
Many of the important functions of biomembranes depend on its fluidity because it determines the translational and rotational motion of lipids and membrane proteins. In this work, we use total internal reflection dark field microscopy (TIRDF Microscopy) to study the lateral and rotational diffusion of gold nanorods (GNR) linked to an artificial supported lipid bilayer.
Streptavidin-conjugated gold nanorods of length 71 nm and diameter of 25 nm were attached to headgroup-biotinylated DOPE lipids in DOPC supported lipid bilayers. GNRs were illuminated with laser light of well-known polarization and their scattered light was detected on a fast camera after separating various polarization components. By monitoring the time-dependent polarization of the detected signal, rotational and lateral diffusion of individual GNRs is imaged. Specifically, we can determine the angular orientation and center of mass position of the rod with microsecond temporal resolution. Using this approach, one can infer information on the physical properties and local dynamic behavior of the membrane such as local viscosity, short-range diffusion, and compositional heterogeneity.