Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.34: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Quantitative phase imaging by focus series reconstruction on non-stained tissue — •Katharina Blessing1,2, Alberto Eljarrat2, Simone Gehrer1, Christoph T. Koch2, and Ana-Sunčana Smith1 — 1PULS-Group, Institiut für theoretische Physik I, Friedrich-Alexander-Universität, Erlangen — 2AG Strukturforschung/Elektronenmikroskopie, Institut für Physik, Humboldt-Universität zu Berlin, Berlin
Many biological objects neither absorb nor scatter light, i.e. cells and tissue are widely invisible for conventional microscopy methods. However, most of their structural information is encoded in the phase. The field of quantitative phase imaging aims to make this information accessible.
Here we introduce a focal series reconstruction approach that only requires a standard optical microscope and computer. The reconstruction is done by a multi-focus transport of intensity equation (MFTIE) algorithm out of a focal series acquired around one in-focus shot. It reconstructs the TIE phase from multiple image pairs and refines it using a short iterative loop. Both steps take partial coherence effects into account in a flux-preserving manner. This algorithm provides amplitude and phase information separately. We tested the approach on human osteosarcoma cells. The applicability of the method to imaging epithelial tissue will be discussed with MDCK II cell colonies used as a model system.