Berlin 2018 – scientific programme
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.39: Poster
Tuesday, March 13, 2018, 14:00–16:00, Poster B
Motion artifact compensation in optical mapping studies with motion by combining marker-free tracking and ratiometric imaging — •Vineesh Kappadan1, Johannes Schröder-Schetelig1, Ulrich Parlitz1, Stefan Luther1,2, and Jan Christoph1,2 — 1Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany — 2German Center for Cardiovascular Research (DZHK e.V.), Göttingen, Germany
Fluorescence imaging or optical mapping provides highly detailed visualizations of cardiac electrophysiology in isolated, intact hearts. Recent developments in optical mapping have opened the path for being able to perform imaging with beating and moving hearts.
Here, we show that marker-free motion tracking and ratiometric imaging can be combined effectively to reduce motion artifacts when filming a beating Langendorff-perfused isolated rabbit heart. We also show that marker-free motion tracking with simultaneous imaging of action potential and calcium transient waves provides a novel tool for investigating the electromechanical dynamics of the heart.
We find that combining motion tracking and ratiometry can significantly enhance motion artifact reduction and allows the comparison and cross-validation of the two techniques with respect to each other.