Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 15: Postersession III
BP 15.43: Poster
Dienstag, 13. März 2018, 14:00–16:00, Poster B
Detecting intracellular-changes below the optical resolution limit to investigate inflammation. — •Florian Schock1,2,3,4, Jan Neumann1,2,3, Anna Lena Leifke2, Ulrich Pöschl2, Kurt Lucas2, and Christoph Cremer1,2,3,4 — 1Institute of Molecular Biology, University of Mainz, Mainz, Germany — 2Max-Planck-Institut for Chemistry Mainz, Germany — 3Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany — 4Kirchhoff Institute for Physics, University of Heidelberg, Heidelberg, Germany
As a result of the interdependency of form and function, microscopy of intracellular structure has become a standard tool to investigate biological processes and medical questions. Hence many projects of the last decades aimed to discover and improve (super-resolution-) light microscopy methods to visualize details far below the classical resolution limit. But it is also possible to use microscopy to identify structures and structural changes without the need to visualize these. Here we want to present and use such a method to investigate the effects of inflammation on mitochondria. Our simulations suggests the possibility to register changes clearly below the resolution limit by analysing wide-field images. We will present the results of an in-vitro study on fibroblasts and the comparison to our simulation. Additionally we will also report on super-resolution methods (SIM and SMLM) to image the mitochondria.