Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 15: Postersession III

BP 15.87: Poster

Dienstag, 13. März 2018, 14:00–16:00, Poster B

Simultaneous cell tracking and visualization of flagellar dynamics of Pseudomonas putida — •Zahra Alirezaei-zanjani, Veronika Waljor, Marius Hintsche, and Carsten Beta — Universität Potsdam, Institut für Physik und Astronomie, Potsdam-Golm, Germany

The soil bacterium Pseudomonas putida propels itself with a polar bundle of helical flagella. It senses changes in its environment and exhibits response of the flagella mediated by a chemosensory system. Our earlier research showed that P. putida exhibits a motion pattern dominated by persistent runs that are interrupted by sharp reversal events (M. Theves, et al. Biophys. J. 2013). Recently, we showed that P. putida may exhibit three different flagellar bundle configurations during swimming: the bundle can push, pull, or wrap around the cell body (M. Hintsche et al. Sci. Rep. 2017, accepted). Here, we present a modified experimental setup that allows us to acquire a large amount of cell trajectories together with information on the bundle configuration for each run. We will use this setup to study the statistics of transitions between the different swimming modes with the ultimate goal to elucidate P. putida’s swimming strategy when navigating in the direction of a nutrition gradient.