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BP: Fachverband Biologische Physik

BP 17: Cell Mechanics I

BP 17.4: Talk

Wednesday, March 14, 2018, 10:15–10:30, H 1058

Magnetogenetic Manipulation of Cellular Functions — •Cornelia Monzel^1,3, Chiara Vicario¹, Domenik Lisse², Elie Balloul¹, Koceila Aizel¹, Mathieu Coppey¹, Jacob Piehler², and Maxime Dahan¹ — ¹Institut Curie, Laboratoire Physico-Chimie, CNRS UMR168, 75005 Paris — ²University of Osnabrück, Department of Biology, 49076 Osnabrück, — ³present address: University of Düsseldorf, Department of Physics, 40225 Düsseldorf

Many cell functions rely on the coordinated activity and spatial distribution of proteins on a subcellular scale. However, few tools are hitherto capable of probing and perturbing intracellular proteins on scales matching their natural spatio-temporal distribution. Here, we develop a novel magnetogenetic approach where intracellular proteins are specifically targeted by magnetic nanoparticles (MNPs) and manipulated with magnetic forces to remotely control individual cell functions. Among these functions are changes in organelle dynamics or the activation of a cell signaling pathway. We demonstrate that semisynthetic MNPs based on the natural iron storage protein ferritin are ideally suited for our magnetic manipulation approach. We explain the MNP design, different means of magnetic stimulation, and show the corresponding biological response.