Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Cell Mechanics I
BP 17.6: Vortrag
Mittwoch, 14. März 2018, 11:30–11:45, H 1058
dynamic RT-DC: time-resolved mechanical single cell analysis at 100 cells / second — •Bob Fregin1, Fabian Czerwinski1, Konstanze Aurich2, Doreen Biedenweg1, and Oliver Otto1 — 1ZIK HIKE, Universität Greifswald, Greifswald, Germany — 2Universitätsklinikum Greifswald, Greifswald, Germany
Real-Time Deformability Cytometry (RT-DC) is a label-free technique for single cell mechanical analysis with high throughput of up to 1,000 cells / second. By observing the shape of cells passing a narrow microfluidic channel their deformation and thus material properties can be quantified. As RT-DC is in the current implementation a single shot technique, i.e. for every single cell only one image at the end of the channel is captured, exclusively time-independent parameters like the elastic modulus can be derived.
Here, we are introducing an extension of RT-DC towards dynamic single cell measurements with the possibility to capture elastic and viscous properties of single cells for up to 100 cells / second. Measurements are carried-out in real-time and allow for monitoring cellular shape-changes along the entire length of the microfluidic channel. By varying the experimental conditions we are capable to access a dynamic range in cell response exceeding one order of magnitude in time.
Dynamic RT-DC on a precursor myeloid cell line HL60 as well as primary cells reveals two characteristic timescales which can be attributed to the non-uniform stress distribution at the inlet compared to the steady-state situation inside the channel. This approach allows to extract model-independent material properties from RT-DC assays.