Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 18: Focus Session: Physics of Microbial Systems - organized by Tobias Bollenbach and Benedikt Sabass
BP 18.11: Vortrag
Mittwoch, 14. März 2018, 12:30–12:45, H 2013
Phenotyping Individual Microbes by Mechanical Stress — •Fabian Czerwinski1, Bob Fregin1, Albert Siryaporn2, and Oliver Otto1 — 1Center for Innovation Competence: Humoral Immune Reactions in Cardiovascular Diseases, University of Greifswald, Germany — 2Department of Physics and Astronomy, University of California Irvine, USA
Microbes typically thrive and prosper in colonies and biofilms, whilst responding very sensitively to their environment. Often, smaller subpopulations take on special tasks that are important for the fate of the bunch. However, probing them individually with high throughput is challenging.
Microfluidics allow for a rapid phenotying of whole bacterial populations ideally capturing individual cells and, therefore, special states. By using the platform of real-time deformability cytometry (RT-DC), e.g., one can distinguish different vegetative states within even huge populations at throughputs beyond 10,000 cells per minute.
We used finite-element simulations to optimize the microfluidic geometries used in RT-DC for geometrical constraints, for flow conditions, and for cellular features. For bacteria, deformation of individual cells as a result of mechanical stress experienced during flow can serve as a distinctive gate.