Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 23: Bioimaging and Biopspectroscopy II

BP 23.1: Vortrag

Mittwoch, 14. März 2018, 15:00–15:15, H 2013

Label-free, real-time monitoring of cytosolic composition and dynamics using digital holographic microscopy — •Daniel Midtvedt, Erik Olsén, Gavin Jeffries, and Fredrik Höök — Chalmers University of Technology

Cells continuously adapt their biophysical properties during their Life cycle as well as in response to changes in the environment. However, quantifying these biophysical changes on single cell level has only recently become possible. In this work we use a digital holographic microscope in combination with a millifluidic chip to study the response of microorganisms and mammalian cells to external stimuli. We demonstrate a label-free quantification of changes in both intracellular osmolarity and macromolecular concentration, on single cell level, in response to a change in medium osmolarity. This platform allows to disentangle cellular water fluxes from production and accumulation of higher molecular weight compounds, which suggests an applicability of this platform in studying a broad range of cellular processes.