Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 23: Bioimaging and Biopspectroscopy II

BP 23.5: Vortrag

Mittwoch, 14. März 2018, 16:00–16:15, H 2013

Cardiac Cells and Heart Tissue Studied by X-ray Imaging and Scanning X-ray Diffraction — •Jan-David Nicolas¹, Marten Bernhardt¹, Susanne Schlick², Malte Tiburcy², Wolfram-Hubertus Zimmermann², Amara Khan³, Frauke Alves³, Karl Toischer⁴, and Tim Salditt¹ — ¹Institut für Röntgenphysik, Friedrich-Hund-Platz 1, 37077 Göttingen — ²Institut für Pharmakologie und Toxikologie, Robert-Koch-Str. 40, 37075 Göttingen — ³Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075 Göttingen — ⁴Klinik für Kardiologie und Pneumologie, Institut für Pharmakologie und Toxologie, Robert-Koch-Str. 40, 37075 Göttingen

We have applied scanning x-ray diffraction on cardiac cells and tissue to characterize the cytoskeletal architecture and to localize potential structural defects due to disease. The method typically involves raster-scanning of a microfocused x-ray beam and collecting scattering patterns at each scan point. The collected data can then be analyzed in view of structural parameters such as the interfilament spacing or orientation of the myosin and actin filaments that are in almost crystalline arrangement in the sarcomere. We will show what signals can be extracted from cells and tissue and how nanoscale architecture develops with differentiation. Since many cardiomyopathies rely on the structural integrity of the sarcomere, the contractile unit of cardiac muscle cells, the present study can be easily extended to characterize cells and tissue from a diseased heart.