Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 27: Cell Mechanics II

BP 27.9: Vortrag

Donnerstag, 15. März 2018, 12:15–12:30, H 1028

Effect of Arp2/3 on 3D migration and cellular mechanical properties — •Stefanie Puder, Tom Kunschmann, and Claudia Tanja Mierke — Biological Physics Division, Peter Debye Institute for Soft Matter Physics, University of Leipzig, Germany

Cellular motility is essential in many physiological processes such as tissue repair during wound healing. The migration of cells in 3D extracellular matrices (ECM) is regulated by the actin cytoskeleton. The actin related protein complex Arp2/3 facilitates nucleation and polymerization of new actin branches, which is supposed to impact cellular mechanical properties. However, whether Arp2/3 affects cellular mechanical properties and subsequently migration of cells is not well understood. We suggested that the Arp2/3 complex facilitates 3D motility into ECM by regulating cellular mechanical properties. Our study focuses on Arp3 conditional knock-down fibroblast cells induced by 4-OH-tamoxifen. Cells are analyzed for their ability to migrate in dense 3D ECM. The knock-down of Arp3 accompanies with a significant reduced invasiveness. Cellular mechanical properties are quantified by an optical cell stretcher and AFM resulting in comparable characteristics of cellular deformability and Young's modulus. We found that Arp3 knock-down cells are less deformable (stiffer) compared to control treated cells in both presented techniques. In conclusion, Arp2/3 complex and its subunit Arp3 are essential for providing mechanical cellular stiffness regulating motility into 3D ECM. We demonstrated that Arp2/3 regulates cellular deformability, stiffness and transmission promoting Arp2/3-dependent cell invasion.