Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 3: Cell Adhesion and Migration, Multicellular Systems I
BP 3.2: Vortrag
Montag, 12. März 2018, 09:45–10:00, H 2013
Organization of Fibronectin and NIH/3T3 Fibroblasts on Bulk Microgrooved TiO2 — •Astrid Weidt1,2, Mareike Zink1, and Stefan G. Mayr2,3 — 1Junior Research Group Biotechnology and Biomedicine, Peter-Debye-Institute for Soft Matter Physics, Leipzig University, Germany — 2Leibniz Institute of Surface Engineering (IOM) e.V., Leipzig, Germany — 3Division of Surface Physics, Leipzig University, Germany
The choice of suitable nano- and microstructures of biomaterials is crucial for successful implant integration within the body. In particular, surface characteristics affect the adsorption of various extra cellular matrix proteins. This work illustrates the interaction of protein adsorption and early cell adhesion on bulk microstructured titanium surfaces with parallel grooves of 27 to 35 micron widths and 15 to 19 micron depths, respectively. In contact with low concentrations of fibronectin solutions, distinct adsorption patterns are observed on the edges of the ridges. Moreover, NIH/3T3 fibroblasts cultured in serum-free medium for 1 h, 3 h and 1 d show enhanced early cell adhesion on fibronectin coated samples compared to uncoated ones. In fact, early adhesion and cell contacts occur mainly on the groove edges where fibronectin adsorption was preferentially detected. Such adsorption patterns also support cellular contact guidance on short time scales which is hardly seen for uncoated samples. Thus, surface structures can promote directed adsorption of low concentrated fibronectin which facilitates early cell adhesion. These results may give rise to new developments in surface engineering of biomedical implants for improved osseointegration.