Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 3: Cell Adhesion and Migration, Multicellular Systems I
BP 3.5: Vortrag
Montag, 12. März 2018, 10:30–10:45, H 2013
Bacterial adhesion under flow condition — •Johannes Mischo1, Friederike Nolle1, Christian Spengler1, Nicolas Thewes1, Markus Bischoff2, and Karin Jacobs1 — 1Department of Experimental Physics, Saarland University, Saarbruecken — 2Institute for Medical Microbiology and Hygiene, Saarland University, Homburg/Saar
Bacterial biofilm formation reduces the effect of antibiotics, which is one of the main reasons for the mandatory removal of infected implants from the body. Therefore, the prevention of biofilm formation or material specifications that result in the death of adhering bacteria without harming somatic cells is considered key in medical implant development. Our flow chamber experiments, as a first step towards in vivo situations, aim at characterizing bacterial adhesion and viability of S. aureus on silicon surfaces. While surface chemistry and subsurface composition of the silicon surfaces are consistent, bacterial adhesion rate and viability on nano-rough silicon can be ascribed to geometry constraints, as changes in the adhesion strength due to a variation of the long-range van der Waals force can be neglected. Comparing adhesion rate and viability on hydrophobic and hydrophilic substrates of identical roughness reveals the influence of short-range, e.g. hydrophobic, forces. The data obtained from our flow chamber measurements can be compared to our single cell force spectroscopy data on the same surfaces.