Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 3: Cell Adhesion and Migration, Multicellular Systems I

BP 3.6: Vortrag

Montag, 12. März 2018, 10:45–11:00, H 2013

Patterning of adhesion mediated by binders of multiple types — •Josip Vlajčević¹ and Ana-Sunčana Smith^1,2 — ¹Rudjer Bošković Institute, Division of Physical Chemistry, Zagreb — ²PULS Group, Institut für Theoretische Physik, Univesität Erlangen-Nürnberg

Cellular adhesion is mediated by binding of multiple proteins of different lengths, flexibilities and binding affinities. However, in most modelling efforts so far, only one type of molecular binding has been considered. These studies showed that the membrane, which in the absence of specific molecular forces resides in a non-specific potential, deforms upon molecular complexation. This in turn introduces cooperative effects that promote further binding.

Building on a coarse grained Monte Carlo framework that quantitatively captures the dynamics of adhesion mediated by a single type of ligand-receptor pairs, we study a complete phase behaviour and the dynamics in the system containing two types of molecular binders which differ in molecular flexibilities, lengths, binding energies and densities.

By including ligand-receptor pairs and flexible polymers that can crosslink both adherent interfaces, we can capture the behaviour observed in experiments with artificially produced DNA-oligomers or the adhesion of T-lymphocyte cells induced by binding of TCR to pMHC and LFA-1 to ICAM-1 proteins during the formation of the immune synapse.