Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 36: Cell Adhesion and Migration, Multicellular Systems II
BP 36.3: Vortrag
Freitag, 16. März 2018, 10:15–10:30, H 1028
Intricate features of 3D cancer cell invasion — •Frank Sauer1, Steffen Grosser2, Josef A. Käs2, and Claudia T. Mierke1 — 1Biological Physics Division, Peter Debye Institute for Soft Matter Physics, University of Leipzig, Germany — 2Soft Matter Physics Division, Peter Debye Institute for Soft Matter Physics, University of Leipzig, Germany
The invasion and the motility of cells into 3D tissues is often connected to cell shape changes and pathologists typically diagnose cancer from cell shape or tissue architecture anomalies. However, the correlation between cell shapes and tissue properties and their influence on cancer cell motility is still not well understood. We developed live invasion assays that allow us to analyze the 3D migration pathway of single cells or cells invading from spheroids into tunable collagen gels on statistically relevant cell numbers. Cell shape information can be correlated with migration patterns, invasion depth and matrix properties. Our results show that the invasiveness and the aspect ratio of single invasive MDA-MB-231 cells is drastically reduced by increasing collagen concentration, whereas clusters of the same cells show a distinct contraction and densification of the collagen network prior to invasion followed by a subsequent degradation. On spheroids from non-invasive MCF7 cells only minor network deformations and no invasion or fiber degradation was observed. Our findings support the view that the mechanical properties of the extracellular matrix are a key factor to initiate cancer cell evasion from cell masses such as spheroids, however, they can also act as an obstacle for single cell migration.