Berlin 2018 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 36: Cell Adhesion and Migration, Multicellular Systems II

BP 36.6: Vortrag

Freitag, 16. März 2018, 11:00–11:15, H 1028

Integrated Heart-on-a-Chip systems: In situ characterization of contractile forces in 3D cardiac µ-tissues — •Oliver Schneider, Stefanie Fuchs, Christopher Probst, and Peter Loskill — Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany

Human induced pluripotent stem (iPS) cells possess the power to revolutionize medicine and basic biological research by opening pathways for individualized medicine and large-scale animal-free drug testing. Over the last years, microfluidic Organ-on-a-Chip (OoC) systems evolved from a conceptual idea to a feasible alternative for animal models. OoCs combine iPS-based tissues with microfabrication technologies to create microphysiological in vitro models featuring human genetic background, physiologically relevant tissue structure and "vasculature-like" perfusion. Most of these systems, however, lack the ability to thoroughly analyze the integrated tissues and their response to administered drugs. We present a novel integrated device enabling the parallelized cultivation and characterization of human cardiac microtissues in a physiological, precisely controlled environment. By combining traction force microscopy with microfluidic OoC-systems, we are able to extract in situ information about the spatial and temporal force distribution in cardiac microtissues. The developed system enables the multiplexed automated analysis of many individualized tissues in parallel, thus bridging the gap from basic tissue creation to viable big data collection.