DPG Phi
Verhandlungen
Verhandlungen
DPG

Berlin 2018 – scientific programme

Parts | Days | Selection | Search | Updates | Downloads | Help

BP: Fachverband Biologische Physik

BP 36: Cell Adhesion and Migration, Multicellular Systems II

BP 36.8: Talk

Friday, March 16, 2018, 11:30–11:45, H 1028

Temperature gradients characterize, counteract and rescue P granule segregation in C. elegans — •Anatol W. Fritsch1, Matthäus Mittasch1, Carsten Hoege1, Frank Jülicher2, Anthony Hyman1, and Moritz Kreysing11MPI-CBG, Dresden, Germany — 2MPI-PKS, Dresden, Germany

Recent studies report on membrane-less condensates in cells, that are formed by liquid-liquid phase separation. In C. elegans zygotes condensates, named P granules, segregate asymmetrically to one daughter cell during the first cell division. This process is involved in the development of a functional germ line. More specifically, the asymmetric localization of P granules depends on a protein gradient of MEX-5 along the long axis of the zygote. MEX-5 in turn, is thought to act through an mRNA competition mechanism and locally regulates the phase separation of the of the condensates.

Using a strategy based on the physical principles of phase-separation, we are able to rescue the asymmetric localization of P granules in MEX-5 RNAi mutants with defective segregation in vivo. We replace the protein gradient with a localized temperature gradient that mimics the physical mechanisms of the local regulation in phase separation. Furthermore, with this approach, we are able to invert the endogenous spatial distribution of P granules in zygotes. This enables us to study the dynamics of in vivo phase separation via controlled physical perturbations. In this study we conclude, that P granule segregation is a spatially tuned, diffusive-flux dependent, evaporation-condensation phenomenon.

100% | Mobile Layout | Deutsche Version | Contact/Imprint/Privacy
DPG-Physik > DPG-Verhandlungen > 2018 > Berlin