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BP: Fachverband Biologische Physik
BP 36: Cell Adhesion and Migration, Multicellular Systems II
BP 36.9: Vortrag
Freitag, 16. März 2018, 11:45–12:00, H 1028
Inversion of rod photoreceptor nuclei improves retinal light transmission by 50% — •Kaushikaram Subramanian1, Martin Weigert1, Heike Petzold1, Marius Ader2, Irina Solovei3, and Moritz Kreysing1 — 1MPI-CBG, Dresden, Germany — 2CRTD, Dresden, Germany — 3LMU, Munich, Germany
Vertebrate retina has a puzzling inverted structure, with 100s of microns of living neural tissue in the light path before its detection by rods and cones. Large number of rods result in densely packed, light scattering nuclei. In nocturnal mammals rods postnatally undergo a hallmark process of nuclear architecture inversion [1]. Previous studies suggest,reduced forward light scattering in isolated rod nuclei and their predicted light focusing was experimentally verified [2].
Now, with high-throughput analysis of wild type and transgenic mouse retina nuclei we establish causality in large angle light scattering and sub nuclear architecture. Using modulation transfer analysis at tissue level, we demonstrate nearly 50% reduction in image detail transfer (Strehl ratio) in transgenic retina lacking nuclear inversion. Modelling and simulation of light propagation reveal a mechanistic relation between single cell scattering and emergent tissue optics. Finally, behavioral studies confirm the visual benefit of inverted nuclear organization for motion detection in low light conditions. Thus, inverted nuclear architecture in mouse retina conclusively improves retinal image transfer and visual function.
References: [1] Solovei et al, Cell(2009) [2] Błaszczak et al, Opt Express(2014) [3] Solovei, et al, Cell(2013)