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BP: Fachverband Biologische Physik

BP 5: Systems Biology & Gene Expression and Signalling

BP 5.2: Vortrag

Montag, 12. März 2018, 15:15–15:30, H 1028

Effect of ultra small carbon nanodots on the gene expression of primary human hematopoietic stem cells — Stefan Fasbender¹, Lisa Zimmermann¹, Ron-Patrick Cadeddu², Rainer Haas², and •Thomas Heinzel¹ — ¹Experimental Condensed Matter Physics, Heinrich-Heine-University Dusseldorf — ²Department of Haematology, Oncology and Clinical Immunology, University Hospital Dusseldorf

Carbon nanodots (CDs) are often considered as nontoxic alternative to inorganic quantum dots. They show potential in a wide range of biomedical applications like long term imaging of normal and malignant cells in vivo and in vitro, cancer diagnostics and therapeutic tumor cell targeting. Here we prepare fluorescent CDs by thermal decomposition of citric acid and diethylentriamine [1] using microwave irradiation. Primary human hematopoietic stem cells (CD34+) obtained from the leukapheresis products of four healthy donors are exposed to a concentration of 500 ug/ml CDs for 36 hours. Via flow cytometry we demonstrate a significant uptake of the particles into the cells and the effect on the gene expression is studied using the Clariom S microarray.

[1] Qu et al., Light: Science & Applications, 2015, 4, e364