Berlin 2018 – scientific programme
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BP: Fachverband Biologische Physik
BP 9: Postersession I
BP 9.22: Poster
Monday, March 12, 2018, 17:30–19:30, Poster A
Uptake and release of proteins in microgels studied on single particle level — •Farzaneh Vaghefikia1, Julia Kratz1, Julia Walter1, Wenjing Xu2, Andrij Pich2, and Jörg Fitter1 — 1RWTH Aachen University, I. Physikalisches Institut (IA), AG Biophysik, Aachen, Germany — 2DWI-Leibniz Institut for Interactive Materials, Aachen, Germany
For efficient drug delivery, a smart network of polymers called microgel particles can be used. These particles with an ability to swell and de-swell in response to environmental changes, allow uptake and release of drugs in a controlled manner. Their sensitivity to environmental changes along with biocompatibility make it a good candidate for a drug carrier, especially for therapeutic proteins. An impact of environmental conditions on proteins outside the cell often causes protein denaturation and aggregation [1]. We studied the uptake and release of the positively charged cytochrome c, in and from microgel particles. The loading of the microgels took place at pH 8, a value at which microgels are charged negatively [2]. By employing wide-field fluorescence microscopy we made a reliable characterization of single particles, e.g. the maximum loading of the particles, the stability of the loading, and the amount of proteins to be released at the target location.
[1] Antosova et al. Trends Biotechnol. 2009
[2] Ricarda Schröder et al, Macromolecules, 2015.