Berlin 2018 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 9: Postersession I
BP 9.26: Poster
Montag, 12. März 2018, 17:30–19:30, Poster A
Cellular uptake of graphene quantum dots into murine precision-cut liver slices and MMTV-PyMT mammary carcinoma cells — •David Kersting1, Stefan Fasbender1, Angelika Hallenberger2, Johanna Naskou3, Katharina Raba4, Johannes Fischer4, Hans Neubauer3, and Thomas Heinzel1 — 1Experimental Condensed Matter Physics, Heinrich-Heine-University Dusseldorf — 2Institute for Anatomy II, University Hospital Dusseldorf — 3Department of Obstetrics and Gynecology, University Hospital Duesseldorf — 4Institute for Transplantation Diagnostics and Cell Therapeutics, University Hospital Dusseldorf
Precision-cut liver slices were produced from outbred C57BL/6 mice liver lobes and incubated under cultural conditions for 1 or 2 days supplementing their growth medium with a defined concentration of N-doped graphene quantum dots (GQDs). The slices were examined using flow cytometry, confocal microscopy and fluorescence spectroscopy clearly indicating a quantum dot uptake into the cultivated tissue, which might decay with increasing penetration depth. These experiments constitute to our knowledge the first incubation of GQDs on precision-cut tissue slices (PCTS), which display a versatile in vitro tool for probing drug effects on cells in a molecular environment close to their in vivo intra-organ situation. In a second approach the GQDs were incubated in two concentrations over a period up to two days on cultured cell lines of the murine MMTV-PyMT mammary carcinoma model. Our first data obtained by flow cytometry hint at a proposed uptake depending linearly on the incubation time.