Berlin 2018 – scientific programme
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BP: Fachverband Biologische Physik
BP 9: Postersession I
BP 9.29: Poster
Monday, March 12, 2018, 17:30–19:30, Poster A
Uptake of fluorescent graphene quantum dots into human breast cancer cell lines — •Rabea Pilch1, Stefan Fasbender1, André Franken2, Marina Willibald2, Katharina Raba3, Johannes Fischer3, Hans Neubauer2, and Thomas Heinzel1 — 1Experimental Condensed Matter Physics, Heinrich-Heine-University Dusseldorf — 2Department of Obstetrics and Gynecology, University Hospital Dusseldorf — 3Institute for Transplantation Diagnostics and Cell Therapeutics, University Hospital Dusseldorf
Fluorescent graphene quantum dots (GQDs) are prepared by thermal decomposition of citric acid and diethylentriamine referring to the method of Qu et al. [1]. The breast cancer cell lines MCF-7 and MDA-MB-231 and the non-tumor cell line MCF-10A are exposed to various concentrations of GQDs for various times. The MTT viability assay shows a decrease of the viability of all three cell lines to 60 % compared to cells without GQDs, when exposed to a maximum concentration of 1 mg/ml GQDs for 72 hours. Flow cytometry is used to analyse the time dependent uptake of the GQDs into the cells. The number of incorporated GQDs per cell is estimated to be 5 billion for the tumor cell lines (MCF-7 and MDA-MB-231) and 43 billion for the non-tumor cell line (MCF-10A) by measuring the fluorescence intensity of cells exposed to GQDs for 48 hours with fluorescence spectroscopy.
[1] Qu et al., Light: Science & Applications, 2015, 4, e364